• Characterization of Novel Extracellular Matrix (Ecm) Proteins (Mgp and Lumican) and Their Implications in Vascular Development, Angiogenesis, and Cancer

      Sharma, Bikram (2013-01-30)
      Extracellular matrix (ECM) constitutes a large component of our tissue structure. Primarily, ECM provides structural and adhesive support to our cells, but it also controls cellular signaling and behavior. Homeostasis of extracellular matrix composition and function is maintained by our body through a balanced synthesis, degradation and remodeling of ECM. However, under pathological conditions and genetic mutations, ECM homeostasis is disrupted due to deregulation in ECM synthesis, assembly, remodeling, and degradation. A number of diseases, including cardiovascular diseases and cancer, are found to occur due to alterations in ECM. Therefore, targeting ECM can be an attractive therapeutic approach to treat these diseases, and it requires our complete understanding of the ECM molecules and the molecular mechanism it employs in controlling cellular functions. To this end, this study is aimed at the characterization of two ECM proteins—Matrix Gla Protein (MGP) and Lumican—for their roles in vascular development, angiogenesis, and cancer. Findings from this study show that MGP is a critical ECM regulator that promotes angiogenic resolution by suppressing endothelial sprouting and stabilizing vascular lumen formation. In addition, MGP also inhibits tumor growth by inhibiting tumor angiogenesis. On the other hand, our findings show Lumican suppresses tumor growth and has anti-angiogenic activity in a context specific manner.
    • Classification of Urban features using Airborne Hyperspectral Data

      Babu, Bharath Ganesh (2010-05-11)
      Accurate mapping and modeling of urban environments are critical for their efficient and successful management. Superior understanding of complex urban environments is made possible by using modern geospatial technologies. This research focuses on thematic classification of urban land use and land cover (LULC) using 248 bands of 2.0 meter resolution hyperspectral data acquired from an airborne imaging spectrometer (AISA+) on 24th July 2006 in and near Terre Haute, Indiana. Three distinct study areas including two commercial classes, two residential classes, and two urban parks/recreational classes were selected for classification and analysis. Four commonly used classification methods – maximum likelihood (ML), extraction and classification of homogeneous objects (ECHO), spectral angle mapper (SAM), and iterative self organizing data analysis (ISODATA) - were applied to each data set. Accuracy assessment was conducted and overall accuracies were compared between the twenty four resulting thematic maps. With the exception of SAM and ISODATA in a complex commercial area, all methods employed classified the designated urban features with more than 80% accuracy. The thematic classification from ECHO showed the best agreement with ground reference samples. The residential area with relatively homogeneous composition was classified consistently with highest accuracy by all four of the classification methods used. The average accuracy amongst the classifiers was 93.60% for this area. When individually observed, the complex recreational area (Deming Park) was classified with the highest accuracy by ECHO, with an accuracy of 96.80% and 96.10% Kappa. The average accuracy amongst all the classifiers was 92.07%. The commercial area with relatively high complexity was classified with the least accuracy by all classifiers. The lowest accuracy was achieved by SAM at 63.90% with 59.20% Kappa. This was also the lowest accuracy in the entire analysis. This study demonstrates the potential for using the visible and near infrared (VNIR) bands from AISA+ hyperspectral data in urban LULC classification. Based on their performance, the need for further research using ECHO and SAM is underscored. The importance incorporating imaging spectrometer data in high resolution urban feature mapping is emphasized.
    • Cloning and Characterization of Hypothetical Exported Proteins from Community Associated Staphylococcus Aureus

      Kaur, Haninder (2013-01-30)
      Methicillin resistant Staphylococcus aureus (MRSA), a major cause of nosocomial infections, has acquired resistance to beta-lactam and other antibiotics. Recently, community associated MRSA (CA-MRSA) has developed independent of hospital associated MRSA (HA-MRSA). One of the major differences between the hospital and community strains is that the former is multi-resistant to antibiotics while the latter is not as resistant but is significantly more invasive. This increased invasiveness and the ability to cause life-threatening infections, even in immunocompetent individuals, makes CA-MRSA critically important as a public health problem. CA-MRSA is known to cause skin and soft tissue infections; bacteria interact with host skin cells and gain access to deeper tissues causing invasive infections. During this process the bacteria may secrete proteins that aid in the interaction with the host by adhering, invading or causing host cell death and lysis. To understand the virulence mechanisms involved in invasion, we investigated genes described as hypothetical proteins in MSSA476. The bioinformatics-selected proteins showed high probability of being secreted and most were unique to CA-MRSA. Our analysis showed 24 such genes. This study shows primer design for 15 of the genes (7 of the 24 had already been cloned in our laboratory). Using gateway cloning, the 15 genes were cloned into BL-21 expression clones. CA-MRSA’s are known for causing invasive skin infections. To further understand the involvement of our proteins of interest in invasion, human keratinocyte cell lines were used in a study of virulence and interaction with skin. To understand the involvement of our hypothetical secreted proteins, we investigated the mRNA expression level, using RT-qPCR and Livak method, of 20 hypothetical exported proteins in presence of human dermal keratinocyte cell line. Our investigation revealed two genes that showed increased mRNA expression in the presence of keratinocytes, which may be due to factors associated with keratinocytes that may have triggered increased mRNA expression. Keratinocytes are capable of forming cell-cell junctions and producing antimicrobial peptides and cytokines in response to microbes. The increased mRNA expression of two genes may be towards binding to junctions for invasion or may be expressed in response to antimicrobial peptides or cytokines.
    • Cloning and Characterization of SAS1738, a Hypothetical Exported Protein from Community-Associated Strain of Staphylococcus Aureus

      Vijaya Kumar, Deepak Kumar (2010-09-22)
      Methicillin resistant Staphylococcus aureus (MRSA) is a group of S. aureus strains that has acquired resistance to a class of beta lactam antibiotics and is the major cause of hospital associated infections. Their discovery goes back to 1960 when the first cases were identified. Recently community associated MRSA infections have emerged and are caused by strains that are independent of those from the hospital environment, related only because they carry some of the same antibiotic resistance genes. Community associated infections (CA) are more severe, producing pus filled lesions that are painful and capable of invasion of deep tissues. Virulence factors comprised of exported proteins are associated with the invasiveness of CA strains. Most of these proteins are hypothetical in nature with unknown function. The aim of this study is to identify and characterize potential virulence factor proteins that may be involved in the infection pathway of CA-MRSA. This study focuses on a unique gene that encodes an exported protein, SAS1738, found on the chromosome of the CA strain MSSA476. The protein SAS1738 was chosen because it is unique to CA strains and has homology to some proteins identified in other S. aureus strains known for their virulence and host immune evasion. The goal of this work is to characterize SAS1738 and to determine its role in the infection pathway of the organism. The gene of interest has been successfully cloned, expressed, and tested for toxicity in Caenorhabditis elegans, a nematode. The toxicity tests showed that SAS1738 is inhibitory to the growth and development of C. elegans. The actual mode of action of this protein in C. elegans is yet to be established. However, location of SAS1738 using a GFP fusion showed that the highest concentration of the fusion protein was in the gut of the worms. The purified protein when tested in a killing assay against C. elegans, resulted in the death of the worms at an average time point of 8 min after treatment. Microbiological assay results showed that the purified SAS1738 possessed antibacterial activity towards Micrococcus luteus and Proteus vulgaris. This suggests that SAS1738 may play a dual role of antagonizing the commensal flora of the human skin such as Micrococcus luteus and also induce a toxic effect on the human cells as suggested by its toxic effect on C. elegans. Determination of the role of this protein in the infection cycle of CA-MRSA will lead to a better understanding of the pathogenicity of the organism and possible development of new treatment strategies.
    • Cognitive,personality and demographic attributes of student change.

      Henry, Jeanne Marie (2012-04-13)
      This study was an examination as to whether cognitive,personality and demographic attributes of students have changed over time.Archival data for 10 coherts of freshman students from a private Midwest engineering institution were used.Data consisted of Myers-Briggs Type Indicator(MBTI) reults,Learning Enviornment Preferences(LEP) results, and demogrpahics.ANOVA's were performed for all interval data and Chi-Square analysess for all nominal data.Statistical significance was found for MBTI Thinking-Perceiving and Sensing-Intution scales,LEP Cognitive Complexity Index scores, and demographic variables including age,parental education level, and SAT scores.Results are discussed in terms of practical significance,trends evidenced in results,implications for further research and educational service provisions.
    • Comparisons of Distributions and Isotopic Geochemistry of Benthic Foraminifera from Seep and Non-seep Environments, Offshore of Costa Rica

      Burkett, Ashley M. (2011-06-17)
      Vertical distribution patterns and stable isotopic geochemistry of benthic foraminifera labeled with CellTracker Green and stained with Rose Bengal were compared at sites of active methane seepage and adjacent non-seep habitats off the Pacific coast of Costa Rica. Sediment cores of bacterial mats from Costa Rica revealed vertical distribution patterns more similar to those seen previously in clam beds, suggesting increased levels of bioturbation compared to nonseep sites. Similar taxa were found at both seep and non-seep sites including: Chilostomella oolina, Uvigerina peregrina and hispida, Cibicides mckannai, and Cassidulina braziliensis. Within active methane seep habitats, elevated substrate such as carbonate rocks, and vestimentiferan tubeworms were examined for living foraminifera. Vestimentiferan tubeworms had highly variable numbers of attached epibenthic foraminifera, dominated by Cibicides wuellerstorfi and Carpenteria monticularis. Stable carbon isotopic comparisons between epibenthic foraminiferal species of Cibicides wuellerstorfi and the vestimentiferan tubeworms on which they reside revealed 10‰ to 30‰ differences between the foraminiferal carbonate and substrate, suggesting that the geochemical signatures of elevated epibenthics were not significantly influenced by the geochemical signature of the substrate on which they reside. This study finds no apparent methane influence on the foraminiferal calcite of elevated epibenthic foraminifera from the three active seep sites studied (Mound 11, Mound 12, and Jaco Scar). This may be because the elevated epibenthics were not exposed to seep-influenced fluids by inhabiting raised substrates. This study also provides a quantitative analysis of coiling directions in elevated epibenthic species at seeps, which has never previously been reported. Statistical analysis revealed that there were no significant differences in isotopic composition between sinstral (left)and dextral (right) coiling Cibicides wuellerstorfi. The results of this study suggest that coiling direction of elevated epibenthic oraminifera, such as Cibicides wuellerstorfi and Carpenteriamonticularis, is a result of biologic factors rather than environmental influences.
    • Dendroclimatic Reconstruction from Bald Cypress in Southwestern Indiana

      Van De Veer, Robin Lyn (2011-09-20)
      In the United States, bald cypress (Taxodium distichum) is generally recognized as an important component of the forested wetlands found in the Southwestern Coastal Plain and the Mississippi River Valley (which extends to the southern Midwest). The lifespan of this deciduous species is important not only commercially, but also in an ecological capacity. This study focuses mainly upon the role the tree plays in its environment and how it can be used as an indicator of climate through drought/flood signals in the rings. Bald cypress is a long-lived tree that can be over 1000 years old and is sensitive to climate and ground water hydrology. Because of these factors it is a favorable choice for dendrochronological study in the region. According to the International Tree-Ring Database, a chronology of the species is not well defined for southwestern Indiana. This research provides this missing information and creates the northern most bald cypress chronology in the Midwest. The study site is located in the extreme southwest of Indiana around Hovey Lake (a backwater lake of the Ohio River) about 10 miles south of Mount Vernon, Indiana. Samples were taken from trees near the shore, both on land and in the water.This study dated some trees to 1855. Analysis of the tree rings, climate data, and river discharge data revealed that bald cypress are not declining in southwestern Indiana. The rate of tree ring growth increases as PDSI does and the rate of river discharge does not seem to affect growth much at all. Even though this is the northernmost bald cypress chronology in the midwest and therefore should be stressed according to the theory of ecological amplitude, this chronology does not fall in the category with the highest series intercorrelation or mean sensitivity. The construction of the dam in 1975 has overwhelmed the climate signal in these trees and the trees continue to be suppressed due to the current water level.
    • Diurnal and nocturnal avian antipredator behavior in thermally challenging conditions

      Carr, Jennie M. (2014-03-18)
      Diurnal avian antipredator behavior has been the focus of much past research, yet the influence of the thermal environment on such behaviors is often overlooked. Far less is known about nocturnal avian antipredator behavior, including how these behaviors are influenced by challenging thermal environments. The first portion of my research focused on how the thermal environment influences the diurnal antipredator behavior of wintering birds while (i) exposed to high wind speeds, (ii) foraging in sunlit and shaded microhabitats, and (iii) when using thermoregulatory postures to conserve body heat. In addition to increasing convective heat loss, high wind speeds increase the prevalence of background movements in the environment. My research demonstrated that wintering sparrows exposed to a moving stimulus are less likely to flush to cover on windy days than on calm days, suggesting that wind-driven visual noise may interfere with predator detection. Predator detection may also vary when feeding in sunlight and shade, and the thermal benefits of foraging in direct sunlight on cold winter days may also play an important role in dictating microhabitat choice. Regardless of the thermal benefits of foraging in sunlight, wintering sparrows preferred to feed in shaded microhabitats even at ambient temperatures well below thermoneutrality. However, these birds foraged in sunlight more frequently as ambient temperatures fell, suggesting a trade-off between thermoregulation (solar input) and predation risk. Additional evidence of such a thermoregulation-predation trade-off was evident in the use of heat-conserving thermoregulatory postures by wintering sparrows. Fluffing the feathers or standing on one foot will reduce the amount of heat lost to the environment. However, such postures slow take-off time and likely result in an increase in predation risk. As such, these risky postures were only used when feeding at relatively low ambient temperatures and when near protective cover. In general, these results indicate that characteristics of the thermal environment play an important role in dictating diurnal antipredator behavior. To address how the thermal environment influences nocturnal avian antipredator behavior, I examined the predation-related costs of using energy-saving nocturnal hypothermia. Many species of birds reduce their nighttime body temperature, thus reducing metabolic rate and conserving energy. Such drops in body temperature may be quite substantial and likely influence a bird’s ability to respond to a potential threat during the night. To examine the potential costs of hypothermia, I conducted nocturnal flight tests on hypothermic mourning doves (Zenaida macroura). In general, doves that cooled by more than 5 °C flew poorly or were unable to fly, but were able to fly well once re-warmed to near their normal daytime body temperatures. Thus, low body temperatures during energy-saving hypothermia likely result in an increase in the risk of nocturnal predation. Nocturnal antipredator behavior was also examined in ruby-throated hummingbirds (Archilochus colubris). These hummingbirds frequently use nocturnal torpor (i.e., deep hypothermia), with significant reductions in body temperature and corresponding inability to respond behaviorally to external stimuli. Although hummingbirds altered torpor use seasonally and over the course of the observation period, hummingbirds did not consistently reduce their use of torpor following an experimental increase in perceived predation risk. Thus, although hypothermia is behaviorally costly, further studies are needed to clarify the role of predation on nocturnal behavior in birds.
    • Effect of Tai Chi on Cardiac Autonomic Function and Salivary Cortisol Level in Healthy Adults.

      Kalsaria, Pratik (2012-05-21)
      Introduction: An estimated 8.2 million American adults (1 in 3) have 1 or more types of cardiovascular disease. Heart rate variability (HRV) analysis is considered a non-invasive procedure for analyzing cardiovascular autonomic influence. Depressed HRV has been linked to stress and abnormal cardiovascular autonomic modulation. Purpose: This study evaluated the acute effects of tai chi on cardiac autonomic function and cortisol level in healthy adults. Design: 10 healthy adults, 7 females and 3 males, with an average age of 54 ± 2.04 were included in this study. They were asked not to consume any alcoholic or caffeinated beverages at least 24 hours before the study. Each subject practiced tai chi for 1 hour. HRV measurements were obtained at supine rest using Nexus biofeedback device before and after 10 min, 20 min and 30 min post tai chi exercise. Saliva samples were collected before tai chi and after 45 min post tai chi practice. Results: The cardiac autonomic function was assessed using frequency domain HRV analyses. Repeated measures ANOVA revealed that there is significant difference in means of HR, SDNN, nLF and nHF between the pre tai chi and post tai chi groups. Using student’s T-test, we found that the nHF increased significantly from 42.79 ± 4.12 to 52.82 ± 4.39 after 30 min post tai chi exercise (p<0.05). In contrast, nLF decreased significantly from 57.21 ± 4.12 to 52.82 ± 4.39 after 30 min post tai chi exercise (p<0.05). HR significantly decreased from 73 ± 2 to 67.79 ± 2.94 after 30 min post tai chi exercise. Also, SDNN increased significantly from 34 ± 5.26 to 41.38 ± 6.42 after 30 min post tai chi exercise. No significant changes in cortisol level were observed between pre tai chi and post tai chi groups. Conclusion: Tai chi exercise can modulate cardiac autonomic tone by enhancing the vagal activity and reducing the sympathetic activity. Long-term beneficial effects of tai chi on cardiac autonomic function need further investigation.
    • Effects of Ectoparasites and Reproductive Class on Roost-Switching and Foraging Behavior of Indiana Bats (Myotis sodalis)

      Ectoparasites of bats have been known to cause harm to their hosts and to affect roost-switching. Little research exists on effects ectoparasites may have on roosting and foraging behavior of the federally endangered Indiana bat (Myotis sodalis). From 2008 through 2010, I collected ectoparasite data and documented roost-switching and foraging behavior of Indiana bats on habitat restoration lands owned by the Indianapolis International Airport (IND) in central Indiana. I tested for differences in roosting and foraging behavior between bats with varying ectoparasite loads, and for differences in ectoparasite load, roost-switching frequency, and foraging behavior between different reproductive classes of Indiana bats. I used the volume of ectoparasites of each Indiana bat when analyzing data. I found a significant difference in roost-switching frequency and ectoparasite volume between reproductive classes. Neither reproductive class nor ectoparasite load significantly affected any aspect of foraging behavior. Indiana bats in this study apparently maintained moderate loads of ectoparasites which may not affect foraging and roosting, but the insignificant results found in this study may have been due to a small sample size. The significant difference in roost-switching between reproductive classes likely demonstrates variation in bat thermoregulation. Lactating females and pregnant females have a higher need for group thermoregulation and switch roosts less frequently than post-lactating females and volant juveniles. Because ectoparasites have been found to increase in maternity colonies, volant juveniles and post-lactating females may disperse from the main colony roost and switch roosts more often to avoid higher intensities of ectoparasites.
    • Environmental Conditions of Green Valley Lake

      Bellamy, Jennifer (2011-03-16)
      Green Valley Lake, western Indiana, has experienced periodic inputs of acid mine drainage (AMD) from the abandoned Green Valley Coal Mine. The lake serves as a state fishing area, and AMD inputs may affect the aquatic ecosystem and human health. The purpose of this research is to determine to what extent the sediments in Green Valley Lake are acting as sinks for metals and if they may impact water quality. Water and bottom sediment samples were taken throughout the lake to evaluate spatial variability of contamination and to determine how the metal concentrations compared to Post Archean Average Shale (PAAS) background values and sediment quality guidelines (SQGs) values. Three cores were taken from the Northwest portion of the lake, where AMD enters via surface flow to evaluate temporal changes in contamination. Results indicate that water quality at Green Valley is acceptable, pH is slightly acidic (pH=6.4) near the locations of AMD input and increases to the east (pH=8.30). The northwest portion of Green Valley Lake is an eutrophic lake, based on its nutrient levels and Secchi disk measurements. Organic matter content, based on LOI, is higher in the older portion of the lake (7-33 wt %) due to the influence of vegetation surrounding the lake. Bottom sediment at Green Valley Lake are acting as a sink for metals and nutrients. Ni and Cd concentrations are above the sediment quality guidelines severe effect level, while Zn and Pb were above the probable effect level. Metals over the severe and probably effect levels should continue to be monitored in the sediments at Green Valley Lake to ensure that organisms are not being impacted.
    • Evaluation of an Avian Radar System

      Gerringer, Michael B. (2014-03-18)
      The problem of bird strikes in aviation is becoming an increasing threat, both to aircraft and to human safety. Management efforts have reduced wildlife hazards below 500 feet and within the immediate airport environment, but traditional methods of monitoring bird activity are limited to an observer’s field of view. Avian radar systems could potentially be useful in monitoring bird activity at great distances from the airport, at higher altitudes, and at night (Dolbeer 2006), but little work has been done to validate the tracking capabilities of avian radar systems. Thus, the goal of this research is to evaluate the detection and tracking abilities of the Merlin Avian Radar System provided by DeTect Inc. Radio-controlled (RC) aircraft flights were used to systematically test the tracking abilities of the Merlin System with respect to distance and altitude. Transits by free-flying birds provided an equally important test of the Merlin System, allowing for the assessment of tracking performance as influenced by flock size, altitude, and distance from the radar unit. Overall tracking performance regarding the RC aircraft and single large bird targets was poor across all study distances and altitudes. However, flocks of large birds such as geese and cranes were tracked well, even those several miles away from the radar unit. Given these results, avian radar could be a useful tool for monitoring bird flock activity at airports, but less so for single large bird targets such as thermalling raptors.
    • Extracellular Matrix Proteins: Implications for Angiogenesis

      Williams, Kent Edward (2010-07-20)
      The extracellular matrix (ECM) is an essential requirement for maintaining permanent shape and rigidity in multicellular organisms. The ECM serves two main functions: scaffolding and signaling. Insoluble collagen and soluble proteoglycans, glycosaminoglycans (GAGs), and glycoproteins allow for water retention and flexibility. The signaling role of the ECM is essential for a multitude of events including vascular development and angiogenesis. Via interactions with vascular endothelial cells, proteins of the ECM can induce or repress angiogenesis.
    • Foraging behavior and seasonal movements of the eastern red bat(Lasiurus Borealis)in Central Indiana.

      Everson, Brianne.L (2012-04-13)
      Twenty-four female Eastern red bats(Lasiurus borealis) were captured and tracked to foraging areas near the Indianapolis International Airport during the summers of 2003 and 2004 with full foraging data obtained on 13. A series of multi-azimuth(3-7) triangulations was used to estimate the location of each bat throughout the night.Euclidean distance analysis was used to examine habitat sue by L.borealis.These bats had smaller home ranges than previously noted as well as smaller homes ranges than other species at this location.They foraged over woodlands,newly planted tree fields,open water,park and pasture lands more than predicted by randomly generated points. They avoided highly urban areas such as commercial lands,gravel pits and transportation corridors more than predicted by randomly generated points.Four female L.borealis were tracked leaving the study site between 15 July and 15 August in 2003 and 2004.Simultaneously,signals were lost on four additional radio-tagged bats.Long-term capture rates of adult L.borealis were examined during 3 netting periods(15 May-15 June,15 June-15 July and 15 July-15 August) from 1998-1999,2002-2004.Nearly twice as many adult L.borealis were captured in the third round of netting compared to the previous two rounnds.Based on a comparison of bats radio-tracked leaving the study area with typical home rage sizes of L.borealis at this site, an increase in lost radio tags,and an increase in capture rates of adult female L.borealis during late summer,it appears that L.borealis begins migrating through the study area in late July.Telemetry data indicate their movement through central Indiana is from east tp west,instead of north to south as idicated in large-scale analyses.
    • Glacial/Interglacial Export Production in the Subantarctic South Pacific

      Adamic, Jessica (2010-07-22)
      Atmospheric CO2 varied considerably in the past; however, the mechanisms that drive this variability are poorly understood. CO2 is linked to marine primary productivity through the biological carbon pump (BCP), leading to hypotheses that past increases in BCP efficiency in areas such as the Southern Ocean may have contributed to glacial CO2 drawdown (Sarmiento & Toggweiler, 1984). Productivity has varied considerably in the past, but the extent, timing, and impacts remain poorly understood. The Subantarctic South Pacific is an area of the ocean that is crucial to the understanding of both glacial climate and paleo-export productivity. Unfortunately, few studies have investigated the central South Pacific because it is so remote. The sediment core MV0502-04JC was recovered from the Subantarctic South Pacific in February-March 2005 at 50°S. The results of this study will be compared with data from ODP Leg 189, Site 1171, also in the Subantarctic South Pacific. Data from these cores will be used to evaluate glacial/interglacial paleo-export production and terrigenous provenance using bulk sediment geochemical proxies, including detailed P geochemistry, P, Ba, and metal elemental ratios, and Baxs. The results of this research suggest each site has variable terrigenous provenance that may have relatively different Fe content. Export production at MV0502-4JC is invariant down core; however, Site 1171 does exhibit glacial/interglacial variations in export production.
    • Holocene Landscape Evolution of the Ohio River Valley from Knob Creek to Rosewood Bottom

      De Rego, Kathryn G. (2012-10-19)
      Rivers and floodplain environments have provided human settlements with resources for thousands of years. By understanding how rivers shape the land around them, we can use landforms to reconstruct ancient fluvial processes and to examine how they have conditioned the way humans interact with their environment. This study investigates the Lower Ohio River during the Holocene. During this period, Midwestern landscapes were adjusting to a variety of processes, including millennial-scale climate change involving the transition from a glacial to an interglacial environment. At the same time, Archaic hunter-gatherers adopted a settlement strategy that favored large river valleys. The cause of this shift has not been resolved. This study examined floodplain sedimentation and change along the Lower Ohio from Knob Creek to Rosewood Bottom through two different scales of analysis: the regional (geologic) scale and within local bottoms. A model of floodplain structure from Madison to Tell City, Indiana was created using a GIS and used to examine differences between geologic regions and Early and Late Holocene sediment. Swales were identified using a DEM, and depth, area, and perimeter-area ratio were used as proxies of their characteristics, which are correlated with backwater environments. Subsurface soil and sediment data were collected from a transect across Rosewood Bottom and within a paleochannel common to the study reach and used to reconstruct floodplain production within an individual bottom. Radiocarbon dates from geologic and archaeological contexts were used to examine Early and Late Holocene sedimentation rates. The Ohio River was active during the Early Holocene. It migrated rapidly and constructed most of the modern floodplain. In some areas, it was anabranching because resistant Pleistocene braid bars formed obstructions to flow that became islands. During the Late Holocene, deposition has been mainly characterized by overbank backwater/slackwater deposition in swales. There is not enough evidence to assign an exogenic cause to this phenomenon. Significant differences in floodplain structure between individual bottoms and regions, the influence of underlying Pleistocene gravels on river migration, and different dates for paleochannel abandonment are indicators of autogenic controls on the Ohio’s behavior and show that bottoms have independent histories. This study complements current models of environmental change for river basins in the Midwest, but it suggests that the stability of the floodplain be given more consideration when analyzing Archaic settlement strategy.